Human coagulation factor V, FV ELISA Kit | MyBioSource MBS701761 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human coagulation factor V, FV ELISA Kit

catalog :

MBS701761

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS701761

products type :

ELISA Kit

products full name :

Human coagulation factor V, FV ELISA Kit

products short name :

[coagulation factor V (proaccelerin, labile factor)]

products name syn :

[Human coagulation factor V (FV) ELISA Kit; FVL; PCCF; activated protein c cofactor; coagulation factor V; coagulation factor V jinjiang A2 domain; factor V Leiden; coagulation factor V (proaccelerin; labile factor)]

other names :

[coagulation factor V; Coagulation factor V; coagulation factor V; factor V Leiden; proaccelerin, labile factor; activated protein c cofactor; coagulation factor V jinjiang A2 domain; coagulation factor V (proaccelerin, labile factor); Activated protein C cofactor; Proaccelerin, labile factorCleaved into the following 2 chains:Coagulation factor V heavy chain; Coagulation factor V light chain]

products gene name :

[F5]

other gene names :

[F5; F5; FVL; PCCF; THPH2; RPRGL1]

uniprot entry name :

FA5_HUMAN

reactivity :

Human

sequence length :

2224

specificity :

This assay has high sensitivity and excellent specificity for detection of rat F1+2. No significant cross-reactivity or interference between rat F1+2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 62.5 pmol/L-4000 pmol/L. Sensitivity: 15.6 pmol/L.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for F1+2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any F1+2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F1+2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F1+2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

105990535

ncbi acc num :

NP_000121.2

ncbi gb acc num :

NM_000130.4

uniprot acc num :

P12259

ncbi mol weight :

251,703 Da

ncbi pathways :

Blood Clotting Cascade Pathway (198840); Common Pathway (106060); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

ncbi summary :

This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008]

uniprot summary :

Function: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. Enzyme regulation: Inhibited by SERPINA5.4 PublicationsManual assertion based on experiment in:Ref.11

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145

more info or order :

MyBioSource product webpage