Human trypsin ELISA Kit | MyBioSource MBS701760 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human trypsin ELISA Kit

catalog :

MBS701760

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS701760

products type :

ELISA Kit

products full name :

Human trypsin ELISA Kit

products short name :

[protease, serine, 1 (trypsin 1)]

products name syn :

[Human trypsin ELISA Kit; MGC120175; MGC149362; TRP1; TRY1; TRY4; TRYP1; cationic trypsinogen; digestive zymogen; nonfunctional trypsin 1; protease; serine; 1; serine protease 1; trypsin I; trypsinogen 1; trypsinogen A; protease; serine; 1 (trypsin 1)]

other names :

[trypsin-1 preproprotein; Trypsin-1; trypsin-1; beta-trypsin; trypsinogen 1; trypsinogen A; digestive zymogen; cationic trypsinogen; nonfunctional trypsin 1; protease, serine, 1 (trypsin 1); Beta-trypsin; Cationic trypsinogen; Serine protease 1; Trypsin ICleaved into the following 2 chains:Alpha-trypsin chain 1; Alpha-trypsin chain 2]

products gene name :

[PRSS1]

other gene names :

[PRSS1; PRSS1; TRP1; TRY1; TRY4; TRYP1; TRP1; TRY1; TRYP1]

uniprot entry name :

TRY1_HUMAN

reactivity :

Human

sequence length :

247

specificity :

This assay has high sensitivity and excellent specificity for detection of human trypsin. No significant cross-reactivity or interference between human trypsin and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma. Detection Range: 62.5 ng/ml -4000 ng/mL. Sensitivity: <15.6 ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for trypsin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any trypsin present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for trypsin is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of trypsin bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4506145

ncbi acc num :

NP_002760.1

ncbi gb acc num :

NM_002769.4

uniprot acc num :

P07477

ncbi mol weight :

26,558 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway (833804); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010)

ncbi summary :

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.1 PublicationManual assertion based on experiment in:Ref.9. Catalytic activity: Preferential cleavage: Arg- -Xaa, Lys- -Xaa. Cofactor: Binds 1 calcium ion per subunit.

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

550

size3 :

96-Strip-Wells

price3 :

795

size4 :

5x96-Strip-Wells

price4 :

2890

size5 :

10x96-Strip-Wells

price5 :

5465

more info or order :

MyBioSource product webpage