ELISA/assay

Rat dopamine, DA ELISA Kit

MBS701755

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Rat dopamine, DA ELISA Kit

[dopamine, DA]

[Rat dopamine; DA ELISA Kit; dopamine; DA]

[DA]

Rat

This assay has high sensitivity and excellent specificity for detection of rat D2D. No significant cross-reactivity or interference between rat D2D and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 7.8 ng/ml-500 ng/ml. Sensitivity: 1.95 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for D2D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any D2D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for D2D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of D2D bound in the initial step. The color development is stopped and the intensity of the color is measured.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

590

96-Strip-Wells

855

5x96-Strip-Wells

3110

10x96-Strip-Wells

5890