product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Indoleamine 2, 3-dioxygenase, IDO ELISA Kit
catalog :
MBS701751
quantity :
48-Strip-Wells
price :
565 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS701751
products type :
ELISA Kit
products full name :
Rat Indoleamine 2, 3-dioxygenase, IDO ELISA Kit
products short name :
indoleamine 2, 3-dioxygenase 1
products name syn :
Rat Indoleamine 2; 3-dioxygenase; IDO ELISA Kit; CD107B; IDO; INDO; indole 2; 3-dioxygenase; indoleamine-pyrrole 2; 3 dioxygenase; indoleamine 2; 3-dioxygenase 1
other names :
indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase 1; indoleamine 2,3-dioxygenase 1; IDO-1; indoleamine 23-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase; indoleamine 2,3-dioxygenase 1; Indoleamine-pyrrole 2,3-dioxygenase
products gene name :
IDO1
other gene names :
Ido1; Ido1; Ido; Indo; Ido; Indo; IDO-1
uniprot entry name :
I23O1_RAT
reactivity :
Rat
sequence length :
407
specificity :
This assay has high sensitivity and excellent specificity for detection of rat IDO. No significant cross-reactivity or interference between rat IDO and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 7.81 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IDO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IDO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IDO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IDO bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
13027384
ncbi acc num :
NP_076463.1
ncbi gb acc num :
NM_023973.1
uniprot acc num :
Q9ERD9
ncbi mol weight :
45,831 Da
ncbi pathways :
African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Tryptophan Metabolism Pathway (83356); Tryptophan Metabolism Pathway (198467); Tryptophan Metabolism Pathway (332)
ncbi summary :
enzyme which catalyzes degradation of L-tryptophan to N-formylkynurenine; human homolog is induced by gamma-interferon and is believed to be involved in that agent's tumor antiproliferative effects [RGD, Feb 2006]
uniprot summary :
Function: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen . Catalytic activity: D-tryptophan + O2 = N-formyl-D-kynurenine. Cofactor: Binds 1 heme group per subunit . Enzyme regulation: Activity is inhibited by and MTH-trp (methylthiohydantoin-DL-tryptophan), modestly inhibited by L-1MT (1-methyl-L-tryptophan) but not D-1MT (1-methyl-D-tryptophan)
size1 :
48-Strip-Wells
price1 :
565 USD
size2 :
96-Strip-Wells
price2 :
810
size3 :
5x96-Strip-Wells
price3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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