ELISA/assay

Human Glucose transporter 4, GLUT4 ELISA Kit

MBS701745

96 Strip Wells

850 USD

ELISA Kit

Human Glucose transporter 4, GLUT4 ELISA Kit

solute carrier family 2 (facilitated glucose transporter) , member 4

Human Glucose transporter 4; GLUT4 ELISA Kit; GLUT4; glucose transporter 4; insulin-responsive glucose transporter type 4; solute carrier family 2 (facilitated glucose transporter); member 4

solute carrier family 2, facilitated glucose transporter member 4; Solute carrier family 2, facilitated glucose transporter member 4; solute carrier family 2, facilitated glucose transporter member 4; GLUT-4; insulin-responsive glucose transporter type 4; glucose transporter type 4, insulin-responsive; solute carrier family 2 (facilitated glucose transporter), member 4; Glucose transporter type 4, insulin-responsive; GLUT-4

SLC2A4

SLC2A4; SLC2A4; GLUT4; GLUT4; GLUT-4

GTR4_HUMAN

Human

509

This assay has high sensitivity and excellent specificity for detection of Human SLC2A4. No significant cross-reactivity or interference between Human SLC2A4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell lysates. Detection Range: 62.5 pg/ml-4000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 15.6 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Sugar transport

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GLUT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLUT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLUT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLUT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4507011

NP_001033.1

NM_001042.2

P14672

54,787 Da

AMPK Signaling Pathway 198868!!AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198832!!Arf6 Trafficking Events Pathway 137954!!Class I PI3K Signaling Events Mediated By Akt Pathway 138020!!Developmental Biology Pathway 477129!!Disease Pathway 530764

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]

Function: Insulin-regulated facilitative glucose transporter.

96 Strip Wells

850 USD