human toll-like receptor 4, TLR4 ELISA Kit | MyBioSource MBS701743 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

human toll-like receptor 4, TLR4 ELISA Kit

catalog :

MBS701743

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701743

products type :

ELISA Kit

products full name :

human toll-like receptor 4, TLR4 ELISA Kit

products short name :

toll-like receptor 4

products name syn :

human toll-like receptor 4; TLR4 ELISA Kit; ARMD10; CD284; TOLL; hToll; homolog of Drosophila toll; toll-like receptor 4

other names :

toll-like receptor 4 isoform C; Toll-like receptor 4; toll-like receptor 4; hToll; homolog of Drosophila toll; toll-like receptor 4; hToll; CD_antigen: CD284

products gene name :

TLR4

other gene names :

TLR4; TLR4; TOLL; CD284; TLR-4; ARMD10

uniprot entry name :

TLR4_HUMAN

reactivity :

Human

sequence length :

799

specificity :

This assay has high sensitivity and excellent specificity for detection of human TLR4. No significant cross-reactivity or interference between human TLR4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human TLR4 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TLR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4507533

ncbi acc num :

NP_003257.1

ncbi gb acc num :

NM_003266.3

uniprot acc num :

O00206

ncbi mol weight :

95,680 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (106400); Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway (187088); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Corticotropin-releasing Hormone Pathway (920957); HIF-1 Signaling Pathway (695200); Hepatitis B Pathway (694606); IKK Complex Recruitment Mediated By RIP1 Pathway (187090)

ncbi summary :

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

Function: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni2+. These responses require non-conserved histidines and are, therefore, species-specific.1 PublicationManual assertion based on experiment in:Ref.17

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage