catalog number :
MBS701719
products type :
ELISA Kit
products full name :
Human anti-mutated citrullinated vimentin, MCV antibody ELISA Kit
products short name :
anti-mutated citrullinated vimentin (MCV) antibody
products name syn :
Human anti-mutated citrullinated vimentin (MCV) antibody ELISA Kit; anti-mutated citrullinated vimentin (MCV) antibody
specificity :
This assay has high sensitivity and excellent specificity for detection of human anti-MCV antibody. No significant cross-reactivity or interference between human anti-MCV antibody and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Detection Range: 5 U/ml -400 U/ml. Sensitivity: The minimum detectable dose of human anti-MCV antibody is typically less than 5 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human anti-MCV antibody concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MCV antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for MCV present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human anti-MCV antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells