catalog number :
MBS701713
products type :
ELISA Kit
products full name :
Chicken Insulin ELISA Kit
products short name :
insulin
products name syn :
Chicken Insulin ELISA kit; ILPR; IRDN; OTTHUMP00000011162; OTTHUMP00000196038; proinsulin; insulin
other names :
insulin preproinsulin; Insulin; insulin; insulin
other gene names :
INS; INS
uniprot entry name :
INS_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of chicken insulin. No significant cross-reactivity or interference between chicken insulin and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 3.12 uIU/ml -50 uIU/ml. Sensitivity: Typically less than 0.59 uIU/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to insulin. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated insulin. A competitive inhibition reaction is launched between insulin (Standards or samples) and Biotin-conjugated insulin with the pre-coated antibody specific for insulin. The more amount of insulin in samples, the less antibody bound by Biotin-conjugated insulin. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of insulin in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_990553.1
ncbi gb acc num :
NM_205222.3
ncbi mol weight :
11,981 Da
ncbi pathways :
FoxO Signaling Pathway (921354); Insulin Signaling Pathway (119320); Insulin Signaling Pathway (501); Oocyte Meiosis Pathway (126917); Oocyte Meiosis Pathway (126872); Progesterone-mediated Oocyte Maturation Pathway (119322); Progesterone-mediated Oocyte Maturation Pathway (503); Regulation Of Actin Cytoskeleton Pathway (119319); Regulation Of Actin Cytoskeleton Pathway (500); Regulation Of Autophagy Pathway (83964)
uniprot summary :
Function: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
size4 :
10x96-Strip-Wells