Human Glycocalicin ELISA Kit | MyBioSource MBS701708 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Glycocalicin ELISA Kit

catalog :

MBS701708

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701708

products type :

ELISA Kit

products full name :

Human Glycocalicin ELISA Kit

products short name :

glycoprotein Ib (platelet) , alpha polypeptide

products name syn :

Human Glycocalicin ELISA kit; BSS; CD42B; CD42b-alpha; GP1B; MGC34595; platelet glycoprotein Ib alpha polypeptide; platelet membrane glycoprotein 1b-alpha subunit; glycoprotein Ib (platelet); alpha polypeptide

other names :

platelet glycoprotein Ib alpha chain; Platelet glycoprotein Ib alpha chain; platelet glycoprotein Ib alpha chain; GP-Ib alpha; antigen CD42b-alpha; platelet membrane glycoprotein 1b-alpha subunit; glycoprotein Ib (platelet), alpha polypeptide; Antigen CD42b-alpha; CD_antigen: CD42bCleaved into the following chain:Glycocalicin

products gene name :

GP1BA

other gene names :

GP1BA; GP1BA; BSS; GP1B; VWDP; CD42B; GPIbA; BDPLT1; BDPLT3; DBPLT3; CD42b-alpha; GP-Ib alpha; GPIb-alpha; GPIbA; Glycoprotein Ibalpha

uniprot entry name :

GP1BA_HUMAN

reactivity :

Human

sequence length :

652

specificity :

This assay has high sensitivity and excellent specificity for detection of human glycocalicin. No significant cross-reactivity or interference between human glycocalicin and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 mug/ml -10 mug/ml. Sensitivity: The minimum detectable dose of human glycocalicin is typically less than 0.039 mug/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for glycocalicin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any glycocalicin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for glycocalicin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of glycocalicin bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

291190772

ncbi acc num :

NP_000164.5

ncbi gb acc num :

NM_000173.5

uniprot acc num :

P07359

ncbi mol weight :

71,540 Da

ncbi pathways :

ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); GP1b-IX-V Activation Signalling Pathway (187205); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (106028); Intrinsic Pathway (106059); Platelet Adhesion To Exposed Collagen Pathway (106030); Platelet Aggregation (Plug Formation) Pathway (106053)

ncbi summary :

Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Mutations in this gene result in Bernard-Soulier syndromes and platelet-type von Willebrand disease. The coding region of this gene is known to contain a polymophic variable number tandem repeat (VNTR) domain that is associated with susceptibility to nonarteritic anterior ischemic optic neuropathy. [provided by RefSeq, Oct 2013]

uniprot summary :

Function: GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage