ELISA/assay

Mouse thymus activation regulated chemokine, TARC ELISA Kit

MBS701706

96 Strip Wells

655 USD

ELISA Kit

Mouse thymus activation regulated chemokine, TARC ELISA Kit

thymus activation regulated chemokine, TARC

Mouse thymus activation regulated chemokine (TARC/CCL17) ELISA Kit; Mouse thymus activation regulated chemokine (TARC/CCL17)

thymus and activation-regulated chemokine; CC chemokine ABCD-2; chemokine (C-C motif) ligand 17; chemokine (C-C motif) ligand 17; small inducible cytokine subfamily A17; chemokine (C-C motif) ligand 17; CC chemokine ABCD-2; Chemokine (C-C motif) ligand 17; Thymus and activation-regulated chemokine

CCL17

Ccl17; Ccl17; Tarc; Abcd-2; Scya17; Scya17l; ABCD-2; TARC; mCG_12286

Q9WUZ6_MOUSE

Mouse

93

This assay has high sensitivity and excellent specificity for detection of mouse TARC. No significant cross-reactivity or interference between mouse TARC and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of mouse thymus activation regulated chemokine (TARC) concentrations in serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 62.5 pg/ml -4000 pg/ml.

Sensitivity: The minimum detectable dose of mouse TARC is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TARC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TARC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARC bound in the initial step. The color development is stopped and the intensity of the color is measured.

5102778

CAB45256.1

Q9WUZ6

10,467 Da

Chemokine Receptors Bind Chemokines Pathway 1000944!!Chemokine Signaling Pathway 99271!!Chemokine Signaling Pathway 672435!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 1000942!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!GPCR Ligand Binding Pathway 1000941!!Peptide Ligand-binding Receptors Pathway 1000943!!Signal Transduction Pathway 970540

96 Strip Wells

655 USD