catalog number :
MBS701698
products type :
ELISA Kit
products full name :
Rat Cortisol ELISA Kit
products short name :
Cortisol
products name syn :
Rat Cortisol ELISA Kit; Rat Cortisol
specificity :
This assay has high sensitivity and excellent specificity for detection of rat cortisol. No significant cross-reactivity or interference between rat cortisol and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Competitive. Detection Range: 0.049 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of rat cortisol is typically less than 0.049 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat cortisol concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for cortisol and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated cortisol and cortisol in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of cortisol in the sample. The color development is stopped and the intensity of the color is measured.
size3 :
10x96-Strip-Wells