catalog number :
MBS701673
products type :
ELISA Kit
products full name :
Mouse Collagen alpha-1 (XVIII) chain, COL18A1/ES ELISA Kit
products short name :
collagen, type XVIII, alpha 1
products name syn :
Mouse Collagen alpha-1 (XVIII) chain (COL18A1/ES) ELISA kit; FLJ27325; FLJ34914; KNO; KNO1; MGC74745; alpha 1 type XVIII collagen; antiangiogenic agent; endostatin; multi-functional protein MFP; collagen; type XVIII; alpha 1
other names :
collagen alpha-1(XVIII) chain isoform 1; Collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; endostatin; procollagen, type XVIII, alpha 1; collagen, type XVIII, alpha 1
products gene name :
COL18A1/ES
other gene names :
Col18a1; Col18a1
uniprot entry name :
COIA1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse COL18A1/ES. No significant cross-reactivity or interference between Mouse COL18A1/ES and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ES has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ES present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ES is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ES bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001103461.1
ncbi gb acc num :
NM_001109991.1
ncbi mol weight :
182,172 Da
ncbi pathways :
Activation Of Matrix Metalloproteinases Pathway (1001029); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1001018); Collagen Biosynthesis And Modifying Enzymes Pathway (1001017); Collagen Degradation Pathway (1001030); Collagen Formation Pathway (1001016); Degradation Of The Extracellular Matrix Pathway (1001028); Extracellular Matrix Organization Pathway (1001015); Integrin Cell Surface Interactions Pathway (1001031); Laminin Interactions Pathway (1001024); PodNet: Protein-protein Interactions In The Podocyte Pathway (755428)
uniprot summary :
Function: Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
size4 :
10x96-Strip-Wells