ELISA/assay

Human Peroxisome Proliferator-activated receptor gamma, PPAR-gamma ELISA Kit

MBS701661

48-Strip-Wells

565 USD

ELISA Kit

Human Peroxisome Proliferator-activated receptor gamma, PPAR-gamma ELISA Kit

peroxisome proliferator-activated receptor gamma

Human Peroxisome Proliferator-activated receptor gamma; PPAR-gamma ELISA Kit; CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARgamma; OTTHUMP00000185030; OTTHUMP00000185033; PPAR gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferative activated receptor gamma; peroxi; peroxisome proliferator-activated receptor gamma

peroxisome proliferator-activated receptor gamma isoform 1; Peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma; PPAR-gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated nuclear receptor gamma variant 1; peroxisome proliferator-activated receptor gamma; Nuclear receptor subfamily 1 group C member 3

PPARG

PPARG; PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma; NR1C3; PPAR-gamma

PPARG_HUMAN

Human

477

This assay has high sensitivity and excellent specificity for detection of Human PPARG. No significant cross-reactivity or interference between Human PPARG and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

Introduction: Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. The PPAR gamma 2 isoform appears to be induced very early in the differentiation of several cultured adipocyte cell lines, and has been suggested to be a dominant regulator of the murine P2 (aP2) gene which encodes an intracellular lipid binding protein which is expressed only in adipose cells. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with RXR alpha. Defects in PPAR gamma can lead to type 2 insulin-resistant diabetes and hyptertension and could play a role in the genetic predisposition to obesity. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPAR-gamma. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PPAR-gamma and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PPAR-gamma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PPAR-gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve.

116284368

NP_005028.4

NM_005037.5

P37231

57,620 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipogenesis Pathway (198832); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Developmental Biology Pathway (477129); Energy Metabolism Pathway (198907); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Huntington's Disease Pathway (83100)

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]

PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 3p25. Cellular Component: nucleoplasm; perinuclear region of cytoplasm; nucleus; cytosol. Molecular Function: ligand-dependent nuclear receptor activity; transcription activator binding; zinc ion binding; drug binding; alpha-actinin binding; protein phosphatase binding; arachidonic acid binding; retinoid X receptor binding; protein binding; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; DNA binding; prostaglandin receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; estrogen receptor binding; chromatin binding; transcription factor activity. Biological Process: heart development; positive regulation of transcription, DNA-dependent; negative regulation of collagen biosynthetic process; cell maturation; rhythmic process; lipid homeostasis; response to lipid; glucose homeostasis; response to vitamin A; response to caffeine; positive regulation of oligodendrocyte differentiation; placenta development; long-chain fatty acid transport; organ regeneration; cell fate commitment; monocyte differentiation; negative regulation of acute inflammatory response; regulation of circadian rhythm; response to starvation; negative regulation of telomerase activity; cellular response to insulin stimulus; response to estrogen stimulus; response to mechanical stimulus; lipoprotein transport; brown fat cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of transcription factor activity; fatty acid oxidation; negative regulation of transcription, DNA-dependent; positive regulation of phagocytosis, engulfment; negative regulation of smooth muscle cell proliferation; low-density lipoprotein receptor biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; epithelial cell differentiation; regulation of blood pressure; response to nutrient; caspase activation; transcription initiation from RNA polymerase II promoter; response to retinoic acid; G-protein coupled receptor protein signaling pathway; response to low density lipoprotein stimulus; white fat cell differentiation; positive regulation of fatty acid oxidation; innate immune response; gene expression; response to cold; lipid metabolic process; negative regulation of cell growth. Disease: Obesity; Lipodystrophy, Familial Partial, Type 3; Carotid Intimal Medial Thickness 1; Diabetes Mellitus, Noninsulin-dependent

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150