ELISA/assay

Human Ceruloplasmin, CP/CER ELISA Kit

MBS701656

48-Strip-Wells

565 USD

ELISA Kit

Human Ceruloplasmin, CP/CER ELISA Kit

ceruloplasmin (ferroxidase)

Human Ceruloplasmin; CP/CER ELISA Kit; CP-2; ceruloplasmin; ceruloplasmin (ferroxidase)

ceruloplasmin; Ceruloplasmin; ceruloplasmin; ceruloplasmin (ferroxidase); Ferroxidase

CP

CP; CP; CP-2

CERU_HUMAN

Human

1065

This assay has high sensitivity and excellent specificity for detection of Human CP. No significant cross-reactivity or interference between Human CP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, urine, saliva. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 2: Anion transport. Protein Biological Process 3: Copper transport

For Sample For the quantitative determination of human ceruloplasmin (CP/CER) concentrations in serum, plasma, cell culture supernates, urine, saliva. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CP bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557485

NP_000087.1

NM_000096.3

P00450

122,205 Da

HIF-1-alpha Transcription Factor Network Pathway (138045); Iron Uptake And Transport Pathway (187191); Metal Ion SLC Transporters Pathway (161066); Porphyrin And Chlorophyll Metabolism Pathway (83021); Porphyrin And Chlorophyll Metabolism Pathway (407); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (119571)

The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]

Function: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe2+ to Fe3+ without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu2+ ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense . Catalytic activity: 4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O. Cofactor: Binds 6 copper ions per monomer.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150