ELISA/assay

Human hepatocyte growth factor activator, HGFA ELISA Kit

MBS701647

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human hepatocyte growth factor activator, HGFA ELISA Kit

[HGF activator]

[Human hepatocyte growth factor activator (HGFA) ELISA kit; RP11-529E10.2; HGFA; MGC138395; MGC138397; ; HGF activator]

[hepatocyte growth factor activator isoform 2 preproprotein; Hepatocyte growth factor activator; hepatocyte growth factor activator; HGF activator]

[HGFAC]

[HGFAC; HGFAC; HGFA; HGF activator; HGFA]

HGFA_HUMAN

Human

655

This assay has high sensitivity and excellent specificity for detection of human HFE. No significant cross-reactivity or interference between human HFE and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HFE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HFE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HFE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HFE bound in the initial step. The color development is stopped and the intensity of the color is measured.

4504383

NP_001519.1

NM_001528.3

Q04756

70,682 Da

This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Function: Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145