catalog number :
MBS701646
products type :
ELISA Kit
products full name :
Rat angiotensin II receptor type 1, AT1R ELISA Kit
products short name :
angiotensin II receptor, type 1
products name syn :
Rat angiotensin II receptor type 1 (AT1R) ELISA Kit; AG2S; AGTR1A; AGTR1B; AT1; AT1B; AT1R; AT2R1; AT2R1A; AT2R1B; HAT1R; angiotensin receptor 1; angiotensin receptor 1B; type-1B angiotensin II receptor; angiotensin II receptor; type 1
other names :
type-1 angiotensin II receptor; Type-1 angiotensin II receptor; type-1 angiotensin II receptor; AT1 ANG II receptor; AT1 angiotensin II receptor; angiotensin II type-1 receptor; Angiotensin II type-1 receptor; AT1
products gene name :
AGTR1
other gene names :
AGTR1; AGTR1; AGTR1A; AT1
uniprot entry name :
AGTR1_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat AGTR1. No significant cross-reactivity or interference between Rat AGTR1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
For samples: For the quantitative determination of rat angiotensin ? receptor type 1 (AT1R) concentrations in serum, plasma, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AT1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AT1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AT1R is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AT1R bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075793.1
ncbi gb acc num :
NM_001082324.1
ncbi mol weight :
40,990 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
size4 :
10x96-Strip-Wells