ELISA/assay

Rat Thyroid Stimulating Hormone, TSH ELISA Kit

MBS701641

96 Strip Wells

725 USD

ELISA Kit

Rat Thyroid Stimulating Hormone, TSH ELISA Kit

Thyroid Stimulating Hormone, TSH

Rat Thyroid Stimulating Hormone; TSH ELISA Kit; Thyroid Stimulating Hormone; TSH

Tsh; Temperature-sensitive hemagglutinin tsh autotransporter; Autotransporter tshCleaved into the following 2 chains:Temperature-sensitive hemagglutinin tsh; Temperature-sensitive hemagglutinin tsh translocator

TSH

tsh

TSH_ECOLX

Rat

1377

This assay has high sensitivity and excellent specificity for detection of Rat TSH. No significant cross-reactivity or interference between Rat TSH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell lysates. Detection Range: 0.6uIU/ml-24uIU/ml. Detection Wavelength: 450 nm. Sensitivity: 0.3uIU/ml

Sample Volume: 50-100ul

Introduction: Thyroid-stimulating hormone (also known as TSH or thyrotropin) is a peptide hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland which regulates the endocrine function of the thyroid gland. TSH stimulates the thyroid gland to secrete the hormones thyroxine (T4) and triiodothyronine (T3).TSH production is controlled by thyrotropin-releasing hormone (TRH), which is manufactured in the hypothalamus and transported to the anterior pituitary gland via the superior hypophyseal artery, where it increases TSH production and release. Somatostatin is also produced by the hypothalamus, and has an opposite effect on the pituitary production of TSH, decreasing or inhibiting its release. The level of thyroid hormones (T3 and T4) in the blood have an effect on the pituitary release of TSH; when the levels of T3 and T4 are low, the production of TSH is increased, and conversely, when levels of T3 and T4 are high, then TSH production is decreased. This effect creates a regulatory negative feedback loop. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TSH. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated monoclonal antibody preparation specific for TSH and incubated. Then substrate solution A and B are added to each well. Only those wells that contain TSH, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

469236

AAA24698.1

Q47692

148,227 Da

Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149

Function: Contributes to the development of lesions and deposition of fibrin in the avian air sacs. It can act both as an adhesin and as a serine protease. Agglutinates erythrocytes while in contact with the extracellular surface of the bacterial cells. Can adhere to purified hemoglobin and bind with great efficiency to extracellular matrix proteins. Cleaves casein and exhibits mucinolytic activity.4 PublicationsManual assertion based on experiment in:Ref.1

96 Strip Wells

725 USD