Human Chitinase-3-like Protein 1, YKL-40/CHI3L1 ELISA Kit | MyBioSource MBS701636 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Chitinase-3-like Protein 1, YKL-40/CHI3L1 ELISA Kit

catalog :

MBS701636

quantity :

96-Strip-Wells

price :

680 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701636

products type :

ELISA Kit

products full name :

Human Chitinase-3-like Protein 1, YKL-40/CHI3L1 ELISA Kit

products short name :

chitinase 3-like 1 (cartilage glycoprotein-39)

products name syn :

Human Chitinase-3-like Protein 1 (YKL-40/CHI3L1) ELISA Kit; ASRT7; DKFZp686N19119; FLJ38139; GP39; HC-gp39; HCGP-3P; YKL40; YYL-40; chitinase 3-like 1; Human Chitinase-3-like Protein 1 (YKL-40/CHI3L1)

other names :

chitinase-3-like protein 1; Chitinase-3-like protein 1; chitinase-3-like protein 1; 39 kDa synovial protein; cartilage glycoprotein 39; chitinase 3-like 1 (cartilage glycoprotein-39); 39 kDa synovial protein; Cartilage glycoprotein 39; CGP-39; GP-39; hCGP-39; YKL-40

products gene name :

CHI3L1

other gene names :

CHI3L1; CHI3L1; GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39; CGP-39; GP-39; hCGP-39

uniprot entry name :

CH3L1_HUMAN

reactivity :

Human

sequence length :

383

specificity :

This assay has high sensitivity and excellent specificity for detection of human YKL-40. No significant cross-reactivity or interference between human YKL-40 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 46.875 pg/ml -3000 pg/ml. Sensitivity: The minimum detectable dose of human YKL-40 is typically less than 78.065 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YKL-40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YKL-40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YKL-40 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YKL-40 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

144226251

ncbi acc num :

NP_001267.2

ncbi gb acc num :

NM_001276.2

uniprot acc num :

P36222

ncbi mol weight :

42,625 Da

ncbi pathways :

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350)

ncbi summary :

Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]

uniprot summary :

CHI3L1: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Belongs to the glycosyl hydrolase 18 family. Protein type: Secreted; Secreted, signal peptide; Cell adhesion. Chromosomal Location of Human Ortholog: 1q32.1. Cellular Component: extracellular space; proteinaceous extracellular matrix; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm. Molecular Function: extracellular matrix structural constituent; chitin binding; chitinase activity; carbohydrate binding. Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; response to mechanical stimulus; apoptosis; cartilage development; carbohydrate metabolic process; activation of NF-kappaB-inducing kinase; immune response; chitin catabolic process; inflammatory response; lung development. Disease: Schizophrenia; Asthma-related Traits, Susceptibility To, 7

size1 :

96-Strip-Wells

price1 :

680 USD

size2 :

5x96-Strip-Wells

price2 :

2140

size3 :

10x96-Strip-Wells

price3 :

3990

more info or order :

MyBioSource product webpage