product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Mast Cell Tryptase, MCT ELISA Kit
catalog :
MBS701634
quantity :
48-Strip-Wells
price :
565 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS701634
products type :
ELISA Kit
products full name :
Rat Mast Cell Tryptase, MCT ELISA Kit
products short name :
tryptase alpha/beta 1
products name syn :
Rat Mast Cell Tryptase; MCT ELISA Kit; TPS1; TPS2; TPSB1; mast cell alpha II tryptase; mast cell beta I tryptase; tryptase alpha II; tryptase beta 1; tryptase beta I; tryptase-I; tryptase-III; tryptase alpha/beta 1
other names :
tryptase; Tryptase; tryptase; MMCP-7; tryptase, skin; tryptase beta 1; mast cell protease 7; tryptase alpha/beta-1; tryptase alpha/beta 1; Mast cell protease 7; rMCP-7; Tryptase alpha/beta-1; Tryptase, skin
products gene name :
TPSAB1
other gene names :
Tpsab1; Tpsab1; Mcpt7; TPSB1; rMCP-7; Mcp7; Mcpt7; Tpsb1; rMCP-7
uniprot entry name :
TRYB1_RAT
reactivity :
Rat
sequence length :
310
specificity :
This assay has high sensitivity and excellent specificity for detection of rat MCT. No significant cross-reactivity or interference between rat MCT and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, urine. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of rat MCT is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCT bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
194239635
ncbi acc num :
NP_062195.2
ncbi gb acc num :
NM_019322.2
uniprot acc num :
P27435
ncbi mol weight :
30,400 Da
ncbi pathways :
Activation Of Matrix Metalloproteinases Pathway (1009937); Degradation Of The Extracellular Matrix Pathway (1009936); Extracellular Matrix Organization Pathway (1009923)
ncbi summary :
serine protease in mast cells [RGD, Feb 2006]
uniprot summary :
Function: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Catalytic activity: Preferential cleavage: Arg- -Xaa, Lys- -Xaa, but with more restricted specificity than trypsin.
size1 :
48-Strip-Wells
price1 :
565 USD
size2 :
96-Strip-Wells
price2 :
810
size3 :
5x96-Strip-Wells
price3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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