ELISA/assay

Human alphaN-acetylglucosaminidase, alphaNAG ELISA Kit

MBS701633

48-Strip-Wells

565 USD

ELISA Kit

Human alphaN-acetylglucosaminidase, alphaNAG ELISA Kit

N-acetylglucosaminidase, alpha-

Human alphaN-acetylglucosaminidase; alphaNAG ELISA Kit; MPS-IIIB; MPS3B; NAG; UFHSD; N-acetyl-alpha-glucosaminidase; alpha-N-acetylglucosaminidase; N-acetylglucosaminidase; alpha-

alpha-N-acetylglucosaminidase; Alpha-N-acetylglucosaminidase; alpha-N-acetylglucosaminidase; N-acetyl-alpha-glucosaminidase; N-acetylglucosaminidase, alpha; N-acetyl-alpha-glucosaminidase; NAG

NAGLU

NAGLU; NAGLU; NAG; MPS3B; UFHSD; MPS-IIIB; UFHSD1; NAG

ANAG_HUMAN

Human

743

This assay has high sensitivity and excellent specificity for detection of human alphaNAG. No significant cross-reactivity or interference between human alphaNAG and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, urine. Assay Type: Competitive. Detection Range: 0. 25 mU/ml -100 mU/ml. Sensitivity: 0.1 mU/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<6%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<11%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for alphaNAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated alphaNAG. A competitive inhibition reaction is launched between alphaNAG (Standards or samples) and HRP-conjugated alphaNAG with the pre-coated antibody specific for alphaNAG. The more amount of alphaNAG in samples, the less antibody bound by HRP-conjugated alphaNAG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of alphaNAG in the sample. The color development is stopped and the intensity of the color is measured.

66346698

NP_000254.2

NM_000263.3

P54802

82,266 Da

Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Degradation Pathway (82981); Glycosaminoglycan Degradation Pathway (355); Glycosaminoglycan Metabolism Pathway (645297); HS-GAG Degradation Pathway (645307); Heparan Sulfate Degradation Pathway (413377); Heparan Sulfate Degradation Pathway (468270); Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway (645304); Lysosome Pathway (99052)

This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq, Jul 2008]

Function: Involved in the degradation of heparan sulfate. Catalytic activity: Hydrolysis of terminal non-reducing N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150