Human P-Selectin ELISA Kit | MyBioSource MBS701631 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human P-Selectin ELISA Kit

catalog :

MBS701631

quantity :

96 Strip Wells

price :

575 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701631

products type :

ELISA Kit

products full name :

Human P-Selectin ELISA Kit

products short name :

P-Selectin

products name syn :

Human P-Selectin ELISA kit; P-Selectin

other names :

P-selectin; P-selectin; P-selectin; GMP-140; granule membrane protein 140; granulocyte membrane protein; CD62 antigen-like family member P; platelet alpha-granule membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein; selectin P (granule membrane protein 140kDa, antigen CD62); CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD_antigen: CD62P

products gene name :

P-Selectin/CD62P

other gene names :

SELP; SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM; GMRP; GRMP; GMP-140; LECAM3; PADGEM

uniprot entry name :

LYAM3_HUMAN

reactivity :

Human

sequence length :

830

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Sample Type: serum, plasma and tissue homogenates. Detection Range: 0.9 ng/ml-60 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.225 ng/ml

other info2 :

Sample Volume: 50-100ul

products description :

Introduction: P-selectin is a cell adhesion molecule (CAM) on the surfaces of activated endothelial cells, which line the inner surface of blood vessels, and activated platelets. In unactivated endothelial cells, it is stored in granules Weibel-Palade bodies, and alpha-granules in unactivated platelets. P-selectin plays an essential role in the initial recruitment of leukocytes (white blood cells) to the site of injury during inflammation. Thrombin is one trigger which can stimulate endothelial-cell release of P-selectin and recent studies suggest an additional Ca2+-independent pathway involved in release of P-selectin. Ligands for P-selectin on eosinophils and neutrophils are similar sialylated, protease-sensitive, endo-beta-galactosidase-resistant structures, clearly different than those reported for E-selectin, and suggest disparate roles for P-selectin and E-selectin during recruitment during inflammatory responses. P-selectin attaches to the actin cytoskeleton through anchor proteins that are still poorly characterized. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-selectin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated polyclonal antibody preparation specific for P-selectin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain P-selectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of P-selectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

157419154

ncbi acc num :

NP_002996.2

ncbi gb acc num :

NM_003005.3

uniprot acc num :

P16109

ncbi mol weight :

90,834 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Hemostasis Pathway 106028!!IL-3 Signaling Pathway 198881!!IL4-mediated Signaling Events Pathway 137933!!Malaria Pathway 152665!!Malaria Pathway 152657!!Platelet Activation, Signaling And Aggregation Pathway 106034!!Platelet Degranulation Pathway 106050

ncbi summary :

This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.1 PublicationManual assertion based on experiment in:Ref.7

size :

96 Strip Wells

price :

575 USD

more info or order :

MyBioSource product webpage