Human coagulation factor X, FX ELISA Kit | MyBioSource MBS701623 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human coagulation factor X, FX ELISA Kit

catalog :

MBS701623

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701623

products type :

ELISA Kit

products full name :

Human coagulation factor X, FX ELISA Kit

products short name :

coagulation factor X

products name syn :

Human coagulation factor X; FX ELISA Kit; RP11-98F14.9; FX; FXA; Stuart-Prower factor; factor Xa; prothrombinase; coagulation factor X

other names :

coagulation factor X preproprotein; Coagulation factor X; coagulation factor X; factor Xa; prothrombinase; Stuart-Prower factor; coagulation factor X; Stuart factor; Stuart-Prower factorCleaved into the following 3 chains:Factor X light chain; Factor X heavy chain; Activated factor Xa heavy chain

products gene name :

F10

other gene names :

F10; F10; FX; FXA

uniprot entry name :

FA10_HUMAN

reactivity :

Human

sequence length :

488

specificity :

This assay has high sensitivity and excellent specificity for detection of Human F10. No significant cross-reactivity or interference between Human F10 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Blood Coagulation. Protein Biological Process 3: Blood coagulation

products description :

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FX. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for FX and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4503625

ncbi acc num :

NP_000495.1

ncbi gb acc num :

NM_000504.3

uniprot acc num :

P00742

ncbi mol weight :

54,732 Da

ncbi pathways :

Blood Clotting Cascade Pathway (198840); Common Pathway (106060); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Extrinsic Pathway (106058); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Gamma-carboxylation Of Protein Precursors Pathway (106233); Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway (106232); Hemostasis Pathway (106028)

ncbi summary :

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. Catalytic activity: Selective cleavage of Arg- -Thr and then Arg- -Ile bonds in prothrombin to form thrombin. Enzyme regulation: Inhibited by SERPINA5 and SERPINA10.2 PublicationsManual assertion based on experiment in:Ref.11

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage