ELISA/assay

Human platelet-derived growth factor CC, PDGF-CC ELISA Kit

MBS701616

96 Strip Wells

760 USD

ELISA Kit

Human platelet-derived growth factor CC, PDGF-CC ELISA Kit

platelet-derived growth factor CC (PDGF-CC)

Human platelet-derived growth factor CC (PDGF-CC) ELISA Kit; platelet-derived growth factor CC (PDGF-CC)

PDGF-CC

Human

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, cell culture supernates, urine, tissue homogenates. Detection Range: 0.156 ng/ml-10 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.039 ng/ml

Sample Volume: 50-100ul

Introduction: The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. PDGF-CC is the third member of the PDGF family discovered after more than two decades of studies on the original members of the family, PDGF-AA and PDGF-BB. Despite the structural similarities of PDGF-CC and the VEGF family, PDGF-CC does not interact with the three VEGF high-affinity receptors (VEGFR-1, VEGFR-2, and VEGFR-3). Instead, the core domain of PDGF-CC specifically interacts with the PDGFR-alphaalpha and PDGFR-alphabeta heterodimeric receptors but not with PDGFR-betabeta homodimers. Similar to PDGF-AA, the core domain of PDGF-CC induces tyrosine phosphorylation of the PDGFR-alphaalpha receptor and cell proliferation of fibroblasts. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDGF-CC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for PDGF-CC and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PDGF-CC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PDGF-CC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

760 USD