ELISA/assay

Mouse matrix metallopeptidase 7 (matrilysin, uterine) , MMP7 ELISA Kit

MBS701613

48-Strip-Wells

465 USD

ELISA Kit

Mouse matrix metallopeptidase 7 (matrilysin, uterine) , MMP7 ELISA Kit

matrix metallopeptidase 7 (matrilysin, uterine)

Mouse matrix metallopeptidase 7 (matrilysin; uterine) (MMP7) ELISA kit; MMP-7; MPSL1; PUMP-1; matrin; matrix metalloproteinase 7; matrix metalloproteinase 7 (matrilysin; uterine) ; uterine matrilysin; matrix metallopeptidase 7 (matrilysin; uterine)

Matrilysin; Matrilysin; matrilysin; MMP-7; matrin; pump-1 protease; matrilysin, uterine; uterine metalloproteinase; matrix metalloproteinase 7; matrix metalloproteinase-7; matrix metallopeptidase 7; Matrin; Matrix metalloproteinase-7; MMP-7; Pump-1 protease; Uterine metalloproteinase

MMP7

Mmp7; Mmp7; MAT; MMP-7

MMP7_MOUSE

Mouse

264

This assay has high sensitivity and excellent specificity for detection of mouse MMP7. No significant cross-reactivity or interference between mouse MMP7 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of mouse MMP7 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

1705981

Q10738.1

Q10738

29,755 Da

Activation Of Matrix Metalloproteinases Pathway (1001029); Alpha6-Beta4 Integrin Signaling Pathway (198373); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1001018); Collagen Degradation Pathway (1001030); Collagen Formation Pathway (1001016); Degradation Of The Extracellular Matrix Pathway (1001028); Extracellular Matrix Organization Pathway (1001015); Matrix Metalloproteinases Pathway (198370); Wnt Signaling Pathway And Pluripotency (198412); Wnt Signaling Pathway (83258)

Function: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase . Catalytic activity: Cleavage of 14-Ala- -Leu-15 and 16-Tyr- -Leu-17 in B chain of insulin. No action on collagen types I, II, IV, V. Cleaves gelatin chain alpha-2(I) > alpha-1(I). Cofactor: Binds 2 calcium ions per subunit

48-Strip-Wells

465 USD

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595