Rat Vasoactive Intestinal Peptide, VIP ELISA Kit | MyBioSource MBS701611 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Vasoactive Intestinal Peptide, VIP ELISA Kit

catalog :

MBS701611

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701611

products type :

ELISA Kit

products full name :

Rat Vasoactive Intestinal Peptide, VIP ELISA Kit

products short name :

vasoactive intestinal peptide

products name syn :

Rat Vasoactive Intestinal Peptide; VIP ELISA Kit; MGC13587; PHM27; ; vasoactive intestinal peptide

other names :

VIP peptides; VIP peptides; Peptide histidine isoleucinamide 27

products gene name :

VIP

other gene names :

VIP; VIP

uniprot entry name :

VIP_RABIT

reactivity :

Rat

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat VIP. No significant cross-reactivity or interference between Rat VIP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Detection Range: 1.56pg/ml-100 pg/ml. Sensitivity: 0.39 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Introduction: Vasoactive intestinal peptide (VIP) is a peptide hormone containing 28 amino acid residues and is produced in many areas of the human body including the gut, pancreas and suprachiasmatic nuclei of the hypothalamus in the brain.It has a half-life in the blood of about two minutes. With respect to the digestive system, VIP seems to induce smooth muscle relaxation (lower esophageal sphincter, stomach, gallbladder), stimulate secretion of water into pancreatic juice and bile, and cause inhibition of gastric acid secretion and absorption from the intestinal lumen. Its role in the intestine is to greatly stimulate secretion of water and electrolytes, as well as dilating intestinal smooth muscle, dilating peripheral blood vessels, stimulating pancreatic bicarbonate secretion, and inhibiting gastrin-stimulated gastric acid secretion. These effects work together to increase motility.It also has the function of stimulating pepsinogen secretion by chief cells. It is also found in the brain and some autonomic nerves. One region of the brain includes a specific area of the suprachiasmatic nuclei (SCN), the location of the master circadian pacemaker. The SCN coordinates daily timekeeping in the body and VIP plays a key role in communication between individual brain cells within this region. Further, VIP is also involved in synchronising the timing of SCN function with the environmental light-dark cycle. Combined, these roles in the SCN make VIP a crucial component of the mammalian circadian timekeeping machinery. VIP helps to regulate prolactin secretion.It is also found in the heart and has significant effects on the cardiovascular system. It causes coronary vasodilation as well as having a positive inotropic and chronotropic effect. Research is being performed to see if it may have a beneficial role in the treatment of heart failure. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VIP. Standards or samples are then added to the appropriate microtiter plate wells with a biotinconjugated antibody preparation specific for VIP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain VIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of VIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

418255

ncbi acc num :

P32649.1

uniprot acc num :

P32649

ncbi mol weight :

8,198 Da

ncbi pathways :

Class B/2 (Secretin Family Receptors) Pathway (106378); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glucagon-type Ligand Receptors Pathway (106380); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

uniprot summary :

Function: VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage