ELISA/assay

Human Serum amyloid A, SAA ELISA Kit

MBS701600

96 Strip Wells

690 USD

ELISA Kit

Human Serum amyloid A, SAA ELISA Kit

serum amyloid A1

Human Serum amyloid A; SAA ELISA Kit; serum amyloid A1

serum amyloid A-1 protein preproprotein; Serum amyloid A-1 protein; serum amyloid A-1 protein; serum amyloid A protein; tumor protein p53 inducible protein 4; serum amyloid A1; Amyloid fibril protein AA

SAA1

SAA1; SAA1; SAA; PIG4; SAA2; TP53I4; SAA

SAA1_HUMAN

Human

122

This assay has high sensitivity and excellent specificity for detection of Human SAA1. No significant cross-reactivity or interference between Human SAA1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, urine. Detection Range: 156ng/ml-10000 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 39 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Acute phase

Introduction: Serum amyloid A (SAA) proteins are a family of apolipoproteins associated with high-density lipoprotein (HDL) in plasma. Different isoforms of SAA are expressed constitutively (constitutive SAAs) at different levels or in response to inflammatory stimuli (acute phase SAAs). These proteins are produced predominantly by the liver. The conservation of these proteins throughout invertebrates and vertebrates suggests that SAAs play a highly essential role in all animals. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SAA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SAA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain SAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

40316912

NP_000322.2

NM_000331.4

P0DJI8

13,532 Da

Activated TLR4 Signalling Pathway 106400!!Advanced Glycosylation Endproduct Receptor Signaling Pathway 187092!!Amyloids Pathway 366238!!Binding And Uptake Of Ligands By Scavenger Receptors Pathway 771599!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytosolic Sensors Of Pathogen-associated DNA Pathway 576255!!DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway 833822!!Disease Pathway 530764!!Folate Metabolism Pathway 198833!!Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway 161021

This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Jun 2012]

Function: Major acute phase protein.

96 Strip Wells

690 USD