catalog number :
MBS701594
products type :
ELISA Kit
products full name :
Rat Collagen alpha-1 (I) chain, COL1A1 ELISA Kit
products short name :
[collagen, type I, alpha 1]
products name syn :
[Rat Collagen alpha-1 (I) chain (COL1A1) ELISA kit; OI4; alpha 1 type I collagen; collagen alpha 1 chain type I; collagen of skin; tendon and bone; alpha-1 chain; pro-alpha-1 collagen type 1; collagen; type I; alpha 1]
other names :
[Collagen alpha-1(I) chain; Collagen alpha-1(I) chain; Alpha-1 type I collagen]
products gene name :
[COL1A1]
other gene names :
[COL1A1]
uniprot entry name :
CO1A1_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of human COL IV. No significant cross-reactivity or interference between human COL IV and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, Plasma, Cell Culture Supernates, Urine. Assay Type: Quantitative Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COL IV has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL IV present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL IV is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL IV bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
4,988 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Type I collagen is a member of group I collagen (fibrillar forming collagen).
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells