Rat factor B, BF ELISA Kit | MyBioSource MBS701586 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat factor B, BF ELISA Kit

catalog :

MBS701586

quantity :

48-Strip-Wells

price :

605 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701586

products type :

ELISA Kit

products full name :

Rat factor B, BF ELISA Kit

products short name :

factor B, BF

products name syn :

Rat factor B; BF ELISA Kit; factor B; BF

other names :

factor B; Complement factor B; complement factor B; C3 proactivator; C3/C5 convertase; C3 proaccelerator; properdin factor B; B-factor, properdin; glycine-rich beta glycoprotein; glycine-rich beta-glycoprotein; complement factor B; C3/C5 convertase; Glycine-rich beta glycoprotein; GBG; PBF2; Properdin factor BCleaved into the following 2 chains:Complement factor B Ba fragment; Complement factor B Bb fragment

products gene name :

other gene names :

CFB; CFB; BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14; BF; BFD; GBG

uniprot entry name :

CFAB_HUMAN

reactivity :

Rat

sequence length :

621

specificity :

This assay has high sensitivity and excellent specificity for detection of rat BF. No significant cross-reactivity or interference between rat BF and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of rat BF is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BF bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

13560705

ncbi acc num :

AAK30167.1

uniprot acc num :

P00751

ncbi mol weight :

68,872 Da

ncbi pathways :

Activation Of C3 And C5 Pathway (106412); Alternative Complement Activation Pathway (106410); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Regulation Of Complement Cascade Pathway (576254); Staphylococcus Aureus Infection Pathway (172846)

ncbi summary :

This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. Catalytic activity: Cleavage of Arg- -Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg- -Xaa bond in complement component C5 alpha-chain to yield C5a and C5b.

size1 :

48-Strip-Wells

price1 :

605 USD

size2 :

96-Strip-Wells

price2 :

870

size3 :

5x96-Strip-Wells

price3 :

2950

size4 :

10x96-Strip-Wells

price4 :

5535

more info or order :

MyBioSource product webpage