ELISA/assay

Human Lipoxin A4, LXA4 ELISA Kit

MBS701583

48-Strip-Wells

565 USD

ELISA Kit

Human Lipoxin A4, LXA4 ELISA Kit

Lipoxin A4, LXA4

Human Lipoxin A4; LXA4 ELISA Kit; Lipoxin A4; LXA4

lipoxin A4 receptor; N-formyl peptide receptor 2; N-formyl peptide receptor 2; RFP; FMLP-R-I; LXA4 receptor; FMLP-related receptor I; formyl peptide receptor-like 1; lipoxin A4 receptor (formyl peptide receptor related); formyl peptide receptor 2; FMLP-related receptor I; FMLP-R-I; Formyl peptide receptor-like 1; HM63; Lipoxin A4 receptor; LXA4 receptor; RFP

LXA4

FPR2; FPR2; ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II; FPRH1; FPRL1; LXA4R; FMLP-R-I; LXA4 receptor

FPR2_HUMAN

Human

351

This assay has high sensitivity and excellent specificity for detection of human LXA4. No significant cross-reactivity or interference between human LXA4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates, cell lysates, urine. Assay Type: Sandwich. Detection Range: 0.625 pg/ml -40 pg/ml. Sensitivity: 0.156 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LXA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LXA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LXA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LXA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

3047269

AAC13684.1

P25090

38,964 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway (161021); G Alpha (i) Signalling Events Pathway (119550); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

Function: Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150