catalog number :
MBS701572
products type :
ELISA Kit
products full name :
Canine Interferon alpha-1/2 ELISA Kit
products short name :
Interferon alpha-1/2
products name syn :
Canine Interferon alpha-1/2 ELISA kit; Interferon alpha-1/2
products gene name :
IFN-alpha1/2
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml-800 pg/ml. Sensitivity: 3.12 pg/ml
other info2 :
Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Interferon Alpha-1/2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Interferon Alpha-1/2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Interferon Alpha-1/2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Interferon Alpha-1/2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
CAA01250.1
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells