ELISA/assay

Mouse Endothelial lipase, EL ELISA Kit

MBS701547

96 Strip Wells

895 USD

ELISA Kit

Mouse Endothelial lipase, EL ELISA Kit

lipase, endothelial

Mouse Endothelial lipase; EL ELISA Kit; EDL; EL; PRO719; endothelial cell-derived lipase; lipase G; lipoprotein lipase H; lipase; endothelial

endothelial lipase; Endothelial lipase; endothelial lipase; EDL; lipose, endothelial; endothelial cell-derived lipase; lipase, endothelial; Endothelial cell-derived lipase; EDL

LIPG

Lipg; Lipg; EL; mEDL; lipase; 3110013K01Rik; EDL

LIPE_MOUSE

Mouse

500

This assay has high sensitivity and excellent specificity for detection of Mouse LIPG. No significant cross-reactivity or interference between Mouse LIPG and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 6.25 ng/ml-400 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 1.56 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Lipid degradation

Introduction: Endothelial lipase (EL) is a newly described member of the triglyceride lipase gene family. It has a considerable molecular homology with lipoprotein lipase (LPL) (44%) and hepatic lipase (HL) (41%). Unlike LPL and HL, this enzyme is synthesized by endothelial cells and functions at the site where it is synthesized. Furthermore, its tissue distribution is different from that of LPL and HL. Unlike LPL and HL, EL is located in the vascular endothelial cells and its expression is highly regulated by cytokines and physical forces, suggesting that it may play a role in the development of atherosclerosis. As a lipase, EL has primarily phospholipase A1 activity. Animals that overexpress EL showed reduced HDL cholesterol levels. Conversely, animals that are deficient in EL showed a marked elevation in HDL cholesterol levels, suggesting that it is a major genetic determinant for the concentration, structure, and metabolism of HDL, which protects against atherosclerosis. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for EL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain EL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of EL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

188219561

NP_034850.3

NM_010720.3

Q9WVG5

56,629 Da

Acylglycerol Degradation Pathway 421788!!Acylglycerol Degradation Pathway 468272!!Glycerolipid Metabolism Pathway 83185!!Glycerolipid Metabolism Pathway 361!!Metabolic Pathways 132962!!Triacylglycerol Degradation Pathway 143022

Function: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin . Catalytic activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate.

96 Strip Wells

895 USD