catalog number :
MBS701545
products type :
ELISA Kit
products full name :
Mouse lipoprotein lipase, LPL ELISA Kit
products short name :
lipoprotein lipase
products name syn :
Mouse lipoprotein lipase; LPL ELISA Kit; HDLCQ11; LIPD; ; lipoprotein lipase
other names :
lipoprotein lipase; Lipoprotein lipase; lipoprotein lipase; O 1-4-5; lipoprotein lipase
other gene names :
Lpl; Lpl; LPL
uniprot entry name :
LIPL_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse LPL. No significant cross-reactivity or interference between mouse LPL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: 0.156 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPL bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_032535.2
ncbi gb acc num :
NM_008509.2
ncbi mol weight :
53,109 Da
ncbi pathways :
Adipogenesis Pathway (198299); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Chylomicron-mediated Lipid Transport Pathway (1001042); Developmental Biology Pathway (970813); Disease Pathway (970606); Diseases Associated With Visual Transduction Pathway (971454); Fatty Acid Beta Oxidation Pathway (198349); Glycerolipid Metabolism Pathway (83185); Glycerolipid Metabolism Pathway (361)
uniprot summary :
Function: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium . Catalytic activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate.
size4 :
10x96-Strip-Wells