Human TNF alpha converting enzyme, TACE ELISA Kit | MyBioSource MBS701544 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human TNF alpha converting enzyme, TACE ELISA Kit

catalog :

MBS701544

quantity :

48-Strip-Wells

price :

450 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701544

products type :

ELISA Kit

products full name :

Human TNF alpha converting enzyme, TACE ELISA Kit

products short name :

ADAM metallopeptidase domain 17

products name syn :

Human TNF alpha converting enzyme; TACE ELISA Kit; ADAM18; CD156B; CSVP; MGC71942; TACE; ADAM metallopeptidase domain 18; TNF-alpha converting enzyme; a disintegrin and metalloprotease domain 17; snake venom-like protease; tumor necrosis factor; alpha; ; ADAM metallopeptidase domain 17

other names :

disintegrin and metalloproteinase domain-containing protein 17 preproprotein; Disintegrin and metalloproteinase domain-containing protein 17; disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; snake venom-like protease; TNF-alpha converting enzyme; ADAM metallopeptidase domain 18; tumor necrosis factor, alpha, converting enzyme; ADAM metallopeptidase domain 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD_antigen: CD156b

products gene name :

ADAM17

other gene names :

ADAM17; ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; CSVP; TACE; ADAM 17

uniprot entry name :

ADA17_HUMAN

reactivity :

Human

sequence length :

824

specificity :

This assay has high sensitivity and excellent specificity for detection of human TACE. No significant cross-reactivity or interference between human TACE and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: 15.6 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TACE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TACE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TACE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TACE bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

73747889

ncbi acc num :

NP_003174.3

ncbi gb acc num :

NM_003183.4

uniprot acc num :

P78536

ncbi mol weight :

93,021 Da

ncbi pathways :

Activated NOTCH1 Transmits Signal To The Nucleus Pathway (576270); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Apoptosis Pathway (105648); BDNF Signaling Pathway (712093); Collagen Degradation Pathway (730309); Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway (771591); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (771595); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (771593)

ncbi summary :

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.4 PublicationsManual assertion based on experiment in:Ref.5. Catalytic activity: Narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala- -Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha). Similarly cleaves other membrane-anchored, cell-surface proteins to 'shed' the extracellular domains. Cofactor: Binds 1 zinc ion per subunit.

size1 :

48-Strip-Wells

price1 :

450 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2435

size4 :

10x96-Strip-Wells

price4 :

4555

more info or order :

MyBioSource product webpage