catalog number :
MBS701541
products type :
ELISA Kit
products full name :
Horse Vascular Endothelial Cell Growth Factor, VEGF ELISA Kit
products short name :
vascular endothelial growth factor A
products name syn :
Horse Vascular Endothelial Cell Growth Factor; VEGF ELISA Kit; RP1-261G23.1; MGC70609; MVCD1; VEGF; VEGF-A; VPF; vascular endothelial growth factor isoform VEGF165; vascular permeability factor; vascular endothelial growth factor A
other names :
vascular endothelial growth factor A; Vascular endothelial growth factor A; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; vascular endothelial growth factor 164; Vascular permeability factor; VPF
products gene name :
VEGFA
other gene names :
VEGFA; VEGFA; VEGF; VEGF-A; VPF
uniprot entry name :
VEGFA_HORSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Horse VEGFA. No significant cross-reactivity or interference between Horse VEGFA and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 31.25 pg/ml-2000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 8 pg/ml
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075290.1
ncbi gb acc num :
NM_001081821.1
ncbi mol weight :
22,312 Da
ncbi pathways :
Bladder Cancer Pathway 90064!!Bladder Cancer Pathway 527!!Cytokine-cytokine Receptor Interaction Pathway 90004!!Cytokine-cytokine Receptor Interaction Pathway 460!!Focal Adhesion Pathway 90020!!Focal Adhesion Pathway 478!!HIF-1 Signaling Pathway 695211!!MicroRNAs In Cancer Pathway 852710!!MicroRNAs In Cancer Pathway 852928!!PI3K-Akt Signaling Pathway 692238
uniprot summary :
Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin