ELISA/assay

Mouse Erythropoietin, EPO ELISA Kit

MBS701540

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Mouse Erythropoietin, EPO ELISA Kit

[erythropoietin]

[Mouse Erythropoietin; EPO ELISA Kit; EP; MGC138142; MVCD2; epoetin; erythropoietin]

[erythropoietin; Erythropoietin; erythropoietin; erythropoietin]

[EPO]

[Epo; Epo]

EPO_MOUSE

Mouse

192

This assay has high sensitivity and excellent specificity for detection of mouse EPO. No significant cross-reactivity or interference between mouse EPO and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates. Detection Range: 31.25 pg/ml -2000 pg/mL. Sensitivity: <7.81 pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPO bound in the initial step. The color development is stopped and the intensity of the color is measured.

21389309

NP_031968.1

NM_007942.2

P07321

21,365 Da

Cellular Response To Hypoxia Pathway (1000311); Cellular Responses To Stress Pathway (971022); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); EPO Receptor Signaling Pathway (198317); HIF-1 Signaling Pathway (695223); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83274); Jak-STAT Signaling Pathway (488)

This gene encodes the glycoprotein hormone erythropoietin that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults. A complete lack of the encoded protein causes embryonic lethal anemia in mice. The conditional inactivation of this gene in adult mice results in a chronic, normocytic and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Function: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145