ELISA/assay

Human epidermal growth factor receptor, EGFR ELISA Kit

MBS701531

96 Strip Wells

605 USD

ELISA Kit

Human epidermal growth factor receptor, EGFR ELISA Kit

epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)

Human epidermal growth factor receptor; EGFR ELISA Kit; ERBB; ERBB1; HER1; PIG61; mENA; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor recept; Human epidermal growth factor receptor; EGFR

epidermal growth factor receptor; Epidermal growth factor receptor; epidermal growth factor receptor; CER; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); epidermal growth factor receptor

EGFR

EGFR; EGFR; c-erbB; CER

EGFR_CHICK

Human

1226

This assay has high sensitivity and excellent specificity for detection of human EGFR. No significant cross-reactivity or interference between human EGFR and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human epidermal growth factor receptor (EGFR) concentrations in serum, plasma, tissue homogenates. Detection Range: 0.312 ng/ml -20 ng/ml.

Sensitivity: The minimum detectable dose of human EGFR is typically less than 0.019 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EGFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound in the initial step. The color development is stopped and the intensity of the color is measured.

158261996

NP_990828.2

NM_205497.2

P13387

77,427 Da

Adherens Junction Pathway 119315!!Adherens Junction Pathway 481!!Androgen Receptor Signaling Pathway 199072!!Calcium Signaling Pathway 83958!!Calcium Signaling Pathway 459!!Cytokine-cytokine Receptor Interaction Pathway 119307!!Cytokine-cytokine Receptor Interaction Pathway 460!!Delta-Notch Signaling Pathway 199057!!Dorso-ventral Axis Formation Pathway 119311!!Dorso-ventral Axis Formation Pathway 472

Function: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Enzyme regulation: Endocytosis and inhibition of the activated EGFR by phosphatases constitute immediate regulatory mechanisms. Moreover, inducible feedback inhibitors may constitute alternative regulatory mechanisms for the EGFR signaling.

96 Strip Wells

605 USD