ELISA/assay

Human beta-subunits of hemoglobin, HB-beta ELISA Kit

MBS701530

48-Strip-Wells

565 USD

ELISA Kit

Human beta-subunits of hemoglobin, HB-beta ELISA Kit

hemoglobin, beta

Human beta-subunits of hemoglobin; HB-beta ELISA Kit; CD113t-C; beta-globin; beta globin; beta globin chain; hemoglobin beta chain; hemoglobin; beta

hemoglobin subunit beta; Hemoglobin subunit beta; hemoglobin subunit beta; beta globin chain; hemoglobin beta chain; hemoglobin, beta; Beta-globin; Hemoglobin beta chainCleaved into the following 2 chains:LVV-hemorphin-7; Spinorphin

HBB

HBB; HBB; CD113t-C; beta-globin

HBB_HUMAN

Human

147

This assay has high sensitivity and excellent specificity for detection of human HB-beta. No significant cross-reactivity or interference between human HB-beta and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, lysate for RBC. Assay Type: Competitive. Detection Range: 7.8 ng/ml -500 ng/ml. Sensitivity: The minimum detectable dose of human HB-beta is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human HB-beta concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for HB-beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated HB-beta. A competitive inhibition reaction is launched between HB-beta (Standards or samples) and biotin-conjugated HB-beta with the pre-coated HB-beta antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of HB-beta bound in the initial step. The color development is stopped and the intensity of the color is measured.

4504349

NP_000509.1

NM_000518.4

P68871

15,998 Da

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway (645347); Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway (645348); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Folate Metabolism Pathway (198833); Hemostasis Pathway (106028); Malaria Pathway (152665); Malaria Pathway (152657)

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]

Function: Involved in oxygen transport from the lung to the various peripheral tissues.2 PublicationsManual assertion based on experiment in:Ref.37

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150