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ELISA/assay

Rat Leptin, LEP ELISA Kit

MBS701500

96 Strip Wells

485 USD

MBS701500

ELISA Kit

Rat Leptin, LEP ELISA Kit

leptin

Rat Leptin; LEP ELISA kit; FLJ94114; OB; OBS; leptin (murine obesity homolog) ; leptin (obesity homolog; mouse) ; obese; mouse; homolog of; obesity factor; leptin

leptin; Leptin; leptin; obesity factor; leptin; Obesity factor

LEP

Lep; Lep; OB; obese; Ob

LEP_RAT

Rat

167

This assay has high sensitivity and excellent specificity for detection of Rat LEP. No significant cross-reactivity or interference between Rat LEP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 0.312 ng/ml-20 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.078 ng/ml

Sample Volume: 50-100ul

Introduction: Leptin (from the Greek leptos, meaning thin) is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately ~16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LEP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for LEP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6981148

NP_037208.1

NM_013076.3

P50596

18,866 Da

AMPK Signaling Pathway 989832!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 83485!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198479!!Cytokine-cytokine Receptor Interaction Pathway 83443!!Cytokine-cytokine Receptor Interaction Pathway 460!!Developmental Biology Pathway 1009870!!Incretin Synthesis, Secretion, And Inactivation Pathway 1009776!!Jak-STAT Signaling Pathway 83469

involved in regulation of body weight, and may have a role in angiogenesis [RGD, Feb 2006]

Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of Lep may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

96 Strip Wells

485 USD