Human Macrophage Inflammatory Protein-3alpha, MIP-3alpha ELISA Kit | MyBioSource MBS701495 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Macrophage Inflammatory Protein-3alpha, MIP-3alpha ELISA Kit

catalog :

MBS701495

quantity :

48-Strip-Wells

price :

450 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701495

products type :

ELISA Kit

products full name :

Human Macrophage Inflammatory Protein-3alpha, MIP-3alpha ELISA Kit

products short name :

chemokine (C-C motif) ligand 20

products name syn :

Human Macrophage Inflammatory Protein-3alpha; MIP-3alpha ELISA kit; CKb4; LARC; MIP-3a; MIP3A; SCYA20; ST38; exodus-1; macrophage inflammatory protein 3 alpha; small inducible cytokine subfamily A (Cys-Cys); member 20; chemokine (C-C motif) ligand 20

other names :

C-C motif chemokine 20 isoform 2; C-C motif chemokine 20; C-C motif chemokine 20; CC chemokine LARC; beta chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20; chemokine (C-C motif) ligand 20; Beta-chemokine exodus-1; CC chemokine LARC; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20Cleaved into the following 3 chains:CCL20(1-67); CCL20(1-64); CCL20(2-70)

products gene name :

CCL20

other gene names :

CCL20; CCL20; CKb4; LARC; ST38; MIP3A; MIP-3a; SCYA20; MIP-3-alpha; LARC; MIP3A; SCYA20; MIP-3-alpha

uniprot entry name :

CCL20_HUMAN

reactivity :

Human

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of Human CCL20. No significant cross-reactivity or interference between Human CCL20 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 2: chemokine. Protein Biological Process 3: Chemotaxis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIP-3alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-3alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIP-3alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIP-3alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

194239688

ncbi acc num :

NP_001123518.1

ncbi gb acc num :

NM_001130046.1

uniprot acc num :

P78556

ncbi mol weight :

10,762 Da

ncbi pathways :

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); EBV LMP1 Signaling Pathway (198790); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020)

ncbi summary :

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

uniprot summary :

Function: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.1 PublicationManual assertion based on experiment in:Ref.8

size1 :

48-Strip-Wells

price1 :

450 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2435

size4 :

10x96-Strip-Wells

price4 :

4555

more info or order :

MyBioSource product webpage