ELISA/assay

Pig Soluble protein-100B, S-100B ELISA Kit

MBS701494

96 Strip Wells

915 USD

ELISA Kit

Pig Soluble protein-100B, S-100B ELISA Kit

Soluble protein-100B, S-100B

Pig Soluble protein-100B; S-100B ELISA Kit; Soluble protein-100B; S-100B

S100 calcium binding protein, beta (neural), isoform CRA_a; Protein S100-B; protein S100-B; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural); S100 calcium binding protein B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B

S-100B

S100B; S100B; NEF; S100; S100-B; S100beta

S100B_HUMAN

Pig

92

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.625 ng/ml-10 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.31 ng/ml

Sample Volume: 50-100ul

For Sample For the quantitative determination of pig soluble protein-100B (S-100B) concentrations in serum, plasma, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-100B. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated S-100B. A competitive inhibition reaction is launched between S-100B (Standards or samples) and Biotin-conjugated S-100B with the pre-coated antibody specific for S-100B. The more amount of S-100B in samples, the less antibody bound by Biotin-conjugated S-100B. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of S-100B in the sample. The color development is stopped and the intensity of the color is measured.

119629675

EAX09270.1

P04271

10,713 Da

Activated TLR4 Signalling Pathway 106400!!Advanced Glycosylation Endproduct Receptor Signaling Pathway 187092!!Cytosolic Sensors Of Pathogen-associated DNA Pathway 576255!!DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway 833822!!Immune System Pathway 106386!!Innate Immune System Pathway 106387!!MyD88 Cascade Initiated On Plasma Membrane Pathway 205107!!MyD88 Dependent Cascade Initiated On Endosome Pathway 187081!!MyD88-independent Cascade Pathway 106401!!MyD88:Mal Cascade Initiated On Plasma Membrane Pathway 106390

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Function: Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.2 PublicationsManual assertion based on experiment in:Ref.10

96 Strip Wells

915 USD