catalog number :
MBS701460
products type :
ELISA Kit
products full name :
Human ReverseTri-iodothyronine, rT3 ELISA Kit
products short name :
ReverseTri-iodothyronine, rT3
products name syn :
Human ReverseTri-iodothyronine; rT3 ELISA Kit; ReverseTri-iodothyronine; rT3
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Sample Type: serum, plasma, cell culture supernates and tissue homogenates. Detection Range: 75 pg/ml-1200 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 37.5pg/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to rT3 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated rT3 and incubated. A competitive inhibition reaction is launched between rT3 (Standards or samples) and Biotin-conjugated rT3 with the pre-coated antibody specific for rT3. The more amount of rT3 in samples, the less antibody bound by Biotin-conjugated T3. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of rT3 in the sample. The color development is stopped and the intensity of the color is measured.