ELISA/assay

Human low-density lipoprotein-receptor-related protein 5, LRP-5 ELISA Kit

MBS701452

96 Strip Wells

850 USD

ELISA Kit

Human low-density lipoprotein-receptor-related protein 5, LRP-5 ELISA Kit

low density lipoprotein receptor-related protein 5

Human low-density lipoprotein-receptor-related protein 5; LRP-5 ELISA Kit; BMND1; EVR1; EVR4; HBM; LR3; LRP7; OPPG; OPS; OPTA1; VBCH2; low density lipoprotein receptor-related protein 7; low density lipoprotein receptor-related protein 5

low-density lipoprotein receptor-related protein 5 isoform 2; Low-density lipoprotein receptor-related protein 5; low-density lipoprotein receptor-related protein 5; low density lipoprotein receptor-related protein 7; low density lipoprotein receptor-related protein 5

LRP5

LRP5; LRP5; HBM; LR3; OPS; EVR1; EVR4; LRP7; OPPG; BMND1; LRP-5; OPTA1; VBCH2; LR3; LRP7; LRP-5

LRP5_HUMAN

Human

1034

This assay has high sensitivity and excellent specificity for detection of Human LRP5. No significant cross-reactivity or interference between Human LRP5 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm

Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Endocytosis

Introduction: LRP5 is involved in the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. Defects in LRP5 are a cause of autosomal dominant and autosomal recessive familial exudative vitreoretinopathy (FEVR). Autosomal dominant FEVR is also referred to as exudative vitreoretinopathy 1 (EVR1); also known as Criswick-Schepens syndrome. FEVR is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. FEVR is reported to have a penetrance of 100%, but clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease-related abnormality is an arc of avascular retina in the extreme temporal periphery. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LRP-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for LRP-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain LRP-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LRP-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

631790829

NP_001278831.1

NM_001291902.1

O75197

179,145 Da

Disease Pathway 530764!!MicroRNAs In Cardiomyocyte Hypertrophy Pathway 198784!!N-cadherin Signaling Events Pathway 137919!!RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway 1016209!!Signal Transduction Pathway 477114!!Signaling By WNT In Cancer Pathway 1016191!!Signaling By Wnt Pathway 106510!!TCF Dependent Signaling In Response To WNT Pathway 980475!!Wnt Signaling Pathway NetPath 198799!!Wnt Signaling Pathway And Pluripotency 198847

This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Function: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Appears be required for postnatal control of vascular regression in the eye. Required for posterior patterning of the epiblast during gastrulation.5 PublicationsManual assertion based on experiment in:Ref.8

96 Strip Wells

850 USD