ELISA/assay

Human Collagen, type V, alpha 1, COL5A1 ELISA Kit

MBS701451

48-Strip-Wells

565 USD

ELISA Kit

Human Collagen, type V, alpha 1, COL5A1 ELISA Kit

collagen, type V, alpha 1

Human Collagen; type V; alpha 1 (COL5A1) ELISA Kit; RP11-263F14.1; alpha 1 type V collagen; collagen; type V; alpha 1

collagen alpha-1(V) chain isoform 1 preproprotein; Collagen alpha-1(V) chain; collagen alpha-1(V) chain; collagen alpha-1(V) chain; collagen, type V, alpha 1

COL5A1

COL5A1; COL5A1

CO5A1_HUMAN

Human

1838

This assay has high sensitivity and excellent specificity for detection of human COL5A1. No significant cross-reactivity or interference between human COL5A1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human COL5A1 is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COL5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL5A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

89276751

NP_000084.3

NM_000093.4

P20908

183,560 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067)

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

Function: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150