product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Agouti Related Protein, AGRP ELISA Kit
catalog :
MBS701428
quantity :
96-Strip-Wells
price :
565 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS701428
products type :
ELISA Kit
products full name :
Human Agouti Related Protein, AGRP ELISA Kit
products short name :
agouti related protein homolog (mouse)
products name syn :
Human Agouti Related Protein; AGRP ELISA Kit; AGRT; ART; ASIP2; MGC118963; agouti related protein; Human Agouti Related Protein; AGRP
other names :
agouti-related protein; Agouti-related protein; agouti-related protein; agouti related protein homolog; agouti related neuropeptide
products gene name :
AGRP
other gene names :
AGRP; AGRP; ART; AGRT; ASIP2; AGRT; ART
uniprot entry name :
AGRP_HUMAN
reactivity :
Human
sequence length :
132
specificity :
This assay has high sensitivity and excellent specificity for detection of Human AGRP. No significant cross-reactivity or interference between Human AGRP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, urine. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: 0.002pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cardiovascular
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AGRP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for AGRP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AGRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AGRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi gi num :
4501995
ncbi acc num :
NP_001129.1
ncbi gb acc num :
NM_001138.1
uniprot acc num :
O00253
ncbi mol weight :
14,440 Da
ncbi pathways :
Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Syndecan-3-mediated Signaling Events Pathway (137952)
ncbi summary :
This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]
uniprot summary :
Function: Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.5 PublicationsManual assertion based on experiment in:Ref.8
size1 :
96-Strip-Wells
price1 :
565 USD
size2 :
5x96-Strip-Wells
price2 :
1725
size3 :
10x96-Strip-Wells
price3 :
3200
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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