ELISA/assay

Human Receptor for advanced glyCation end products, RAGE/AGER ELISA Kit

MBS701427

96 Strip Wells

760 USD

ELISA Kit

Human Receptor for advanced glyCation end products, RAGE/AGER ELISA Kit

advanced glycosylation end product-specific receptor

Human Receptor for advanced glycation end products; RAGE/AGER ELISA Kit; DAMA-358M23.4; MGC22357; RAGE; receptor for advanced glycosylation end-products; advanced glycosylation end product-specific receptor

advanced glycosylation end product-specific receptor isoform 1; Advanced glycosylation end product-specific receptor; advanced glycosylation end product-specific receptor; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta; receptor for advanced glycation end-products variant 20; advanced glycosylation end product-specific receptor; Receptor for advanced glycosylation end products

AGER

AGER; AGER; RAGE; RAGE

RAGE_HUMAN

Human

404

This assay has high sensitivity and excellent specificity for detection of Human AGER. No significant cross-reactivity or interference between Human AGER and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 78 pg/ml-5000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 19.5 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Inflammatory response

Introduction: RAGE, the receptor for advanced glycation endproducts is a 35kD transmembrane receptor of the immunoglobulin super family which was first characterized in 1992 by Neeper et al.[1]. Its name comes from its ability to bind advanced glycation endproducts (AGE), a heterogeneous group of non-enzymatically altered proteins. Besides AGEs, RAGE is also able to bind other ligands and is thus often referred to as a pattern recognition receptor. The interaction between RAGE and its ligands is thought to result in pro-inflammatory gene activation[2]. Due to an enhanced level of RAGE ligands in diabetes or other chronic disorders, this receptor is hypothesised to have a causative effect in a range of inflammatory diseases such as diabetic complications, Alzheimer's disease and even some tumors. Isoforms of the RAGE protein, which lack the transmembrane and the signalling domain (commonly referred to as soluble RAGE or sRAGE) are hypothesized to counteract the detrimental action of the full-length receptor and are hoped to provide a means to develop a cure against RAGE-associated diseases. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAGE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for RAGE and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain RAGE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of RAGE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

10835203

NP_001127.1

NM_001136.4

Q15109

42,803 Da

AGE/RAGE Pathway 698754!!Activated TLR4 Signalling Pathway 106400!!Advanced Glycosylation Endproduct Receptor Signaling Pathway 187092!!Cytosolic Sensors Of Pathogen-associated DNA Pathway 576255!!DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway 833822!!Immune System Pathway 106386!!Innate Immune System Pathway 106387!!MyD88 Cascade Initiated On Plasma Membrane Pathway 205107!!MyD88 Dependent Cascade Initiated On Endosome Pathway 187081!!MyD88-independent Cascade Pathway 106401

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]

Function: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.4 PublicationsManual assertion based on experiment in:Ref.15

96 Strip Wells

760 USD