ELISA/assay

Fish growth hormone, GH ELISA Kit

MBS701414

48-Strip-Wells

605 USD

ELISA Kit

Fish growth hormone, GH ELISA Kit

growth hormone, GH

Fish growth hormone; GH ELISA Kit; growth hormone; GH

growth hormone; Somatotropin; somatotropin; pituitary growth hormone; growth hormone 1; Growth hormone; GH; GH-N; Growth hormone 1; Pituitary growth hormone

GH

GH1; GH1; GH; GHN; GH-N; hGH-N; IGHD1B; GH; GH-N

SOMA_HUMAN

Fish

217

This assay has high sensitivity and excellent specificity for detection of fish GH. No significant cross-reactivity or interference between fish GH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 312.5 pg/ml - 5000 pg/ml. Sensitivity: 312.5 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GH. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated GH. A competitive inhibition reaction is launched between GH (Standards or samples) and Biotin-conjugated GH with the pre-coated antibody specific for GH. The more amount of GH in samples, the less antibody bound by Biotin-conjugated GH. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of GH in the sample. The color development is stopped and the intensity of the color is measured.

183157

AAA98618.1

P01241

20,201 Da

Adipogenesis Pathway (198832); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endochondral Ossification Pathway (198812); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Metabolism Of Proteins Pathway (106230)

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul 2008]

Function: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

48-Strip-Wells

605 USD

96-Strip-Wells

870

5x96-Strip-Wells

2950

10x96-Strip-Wells

5535