ELISA/assay

Human leukocyte antigen G, HLA-G ELISA Kit

MBS701413

48-Strip-Wells

510 USD

ELISA Kit

Human leukocyte antigen G, HLA-G ELISA Kit

major histocompatibility complex, class I, G

Human leukocyte antigen G; HLA-G ELISA Kit; DADB-15K14.8; MHC-G; HLA class I molecule; HLA-G histocompatibility antigen; class I; G; MHC class I antigen; b2 microglobulin; major histocompatibility complex; class I; G

HLA class I histocompatibility antigen, alpha chain G; HLA class I histocompatibility antigen, alpha chain G; HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; b2 microglobulin; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G; major histocompatibility complex, class I, G; HLA G antigen; MHC class I antigen G

HLA-G

HLA-G; HLA-G; MHC-G; HLA-6.0; HLAG

HLAG_HUMAN

Human

338

This assay has high sensitivity and excellent specificity for detection of human HLA-G. No significant cross-reactivity or interference between human HLA-G and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml-80 ng/ml. Sensitivity: Typically less than 0.31 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HLA-G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HLA-G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HLA-G is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HLA-G bound in the initial step. The color development is stopped and the intensity of the color is measured.

4504415

NP_002118.1

NM_002127.5

P17693

38,224 Da

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Function: Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800