catalog number :
MBS701409
products type :
ELISA Kit
products full name :
human Fetuin A ELISA Kit
products short name :
alpha-2-HS-glycoprotein
products name syn :
human Fetuin A ELISA Kit; A2HS; AHS; FETUA; HSGA; fetuin-A; alpha-2-HS-glycoprotein
other names :
alpha-2-HS-glycoprotein preproprotein; Alpha-2-HS-glycoprotein; alpha-2-HS-glycoprotein; fetuin-A; alpha-2-Z-globulin; ba-alpha-2-glycoprotein; alpha-2-HS-glycoprotein; Alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; Fetuin-ACleaved into the following 2 chains:Alpha-2-HS-glycoprotein chain A; Alpha-2-HS-glycoprotein chain B
products gene name :
AHSG
other gene names :
AHSG; AHSG; AHS; A2HS; HSGA; FETUA; FETUA; PRO2743
uniprot entry name :
FETUA_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human fetuin A. No significant cross-reactivity or interference between human fetuin A and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates, urine. Assay Type: Sandwich. Detection Range: 15.6 ng/ml -1000 ng/ml. Sensitivity: The minimum detectable dose of human fetuin A is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for fetuin A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any fetuin A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for fetuin A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of fetuin A bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001613.2
ncbi gb acc num :
NM_001622.2
ncbi mol weight :
39,325 Da
ncbi pathways :
BMP Receptor Signaling Pathway (137948)
ncbi summary :
Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
size4 :
10x96-Strip-Wells