catalog number :
MBS701383
products type :
ELISA Kit
products full name :
Human Complement fragment 3b, C3b ELISA Kit
products short name :
Complement fragment 3b, C3b
products name syn :
Human Complement fragment 3b; C3b ELISA Kit; Complement fragment 3b; C3b
other names :
complement component c3a, duplicate 2; Complement component C3-1; complement component c3a, duplicate 2; c3.2; complement component c3b; complement component C3-1; complement component c3a, duplicate 2; Complement component C3-1
other gene names :
c3a.2; c3b; c3b; C3-1; C3-2; C3-1
uniprot entry name :
Q3MU74_DANRE
specificity :
This assay has high sensitivity and excellent specificity for detection of human C3b. No significant cross-reactivity or interference between human C3b and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, urine, cerebrospinal fluid (CSF), saliva. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of human C3b is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C3b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C3b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C3b is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C3b bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_571318.1
ncbi gb acc num :
NM_131243.1
ncbi mol weight :
184,757 Da
ncbi pathways :
Herpes Simplex Infection Pathway (377867); Herpes Simplex Infection Pathway (377865); Phagosome Pathway (153899); Phagosome Pathway (153859)
size4 :
10x96-Strip-Wells