catalog number :
MBS701365
products type :
ELISA Kit
products full name :
Rat Tissue factor pathway inhibitor, TFPI ELISA Kit
products short name :
[tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)]
products name syn :
[Rat Tissue factor pathway inhibitor; TFPI ELISA Kit; EPI; LACI; TFI; TFPI1; OTTHUMP00000163472; OTTHUMP00000205431; anti-convertin; extrinsic pathway inhibitor; lipoprotein-associated coagulation inhibitor; tissue factor pathway inhibitor; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)]
other names :
[tissue factor pathway inhibitor; Tissue factor pathway inhibitor; tissue factor pathway inhibitor; extrinsic pathway inhibitor; lipoprotein-associated coagulation inhibitor; Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI]
products gene name :
[TFPI]
other gene names :
[TFPI; TFPI; EPI; LACI; TFPI; EPI; LACI]
uniprot entry name :
TFPI1_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat TFPI. No significant cross-reactivity or interference between rat TFPI and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 7.8 ng/ml -500 ng/ml. Sensitivity: Typically less than 1.95 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TFPI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TFPI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TFPI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TFPI bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001095184.1
ncbi gb acc num :
NM_001101714.1
ncbi mol weight :
34,436 Da
ncbi pathways :
Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Extrinsic Pathway (106058); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028); Syndecan-4-mediated Signaling Events Pathway (138064)
uniprot summary :
Function: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells